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CABRI:NCCB_PHAGES |
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Collection_number |
NCCB 3173
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Name |
mGP1-2
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Type |
phage
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Other_collection_numbers |
PC-V3173
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Restricted_distribution |
Yes
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Literature |
Tabor, S., (1990), Current protocols in molecular biology (F.A. Ausubel et al., eds) pp.
16.2.1- 16.2.11. Greene publishing and Wiley-Interscience, New York
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History_of_deposit |
NCCB <- Tabor
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Host_for_propagation |
male E. coli strain, carrying an amber suppressor (e.g. MV1190, 71.18, JM101)
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Host_used_for_propagation |
-
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Lysogenicity |
No
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Virus_used_for |
-
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Cell_surface_receptor |
F-pili
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Medium |
Medium 240
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Class |
Recombinant
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Constructed_from |
M13mp8
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Plaque_forming_phage |
No
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Clear_plaque_morphology |
No
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Nucleic_acid_type |
DNA,single stranded,circular
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Properties_and_applications |
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Cloned_gene |
T7 RNA polymerase gene behind the Plac
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Further_information |
induction of T7-polymerase can be done in any male strain, induction in MV1190
however is found to be better than in K38 (suppressor-free). An m.o.i. of 10 is used for induction
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Price_code |
1
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